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Paper I — 2024 December (Supplementary) (2019 Scheme) — Qxi

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Paper I
2024 December (Supplementary) (2019 Scheme) · 100 marks · 180 min

Question

Which of the following can be used to cultivate viruses

  • a) Blood agar
  • b) Thioglycollate broth
  • c) Tissue culture
  • d) Stuart’s medium
Qxi1 marksMCQs

Answer

- [x] c) Tissue culture

Viruses are obligate intracellular parasites and can only be propagated within living host cells — tissue (cell) culture is one of the classical methods for cultivating viruses (alongside embryonated eggs and laboratory animal inoculation), used for isolation, cytopathic effect study, and vaccine production. Blood agar and thioglycollate broth support the growth of bacteria (the latter particularly anaerobes) but not viruses, which lack independent metabolic machinery. Stuart’s medium is a bacterial transport medium (for swabs), not a growth medium of any kind, and cannot support viral or bacterial replication.

  1. 1General Properties of Viruses and Virus Host Interaction

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