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Paper I — 2025 August (Supplementary) (2010 Scheme) — Q5

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Paper I
2025 August (Supplementary) (2010 Scheme) · 40 marks · 120 min

Question

Define enriched media with two example

Q52 marksShort Notes

Answer

Enriched media are basal media supplemented with extra growth factors — whole blood, lysed blood, serum, or egg — that fastidious organisms need but ordinary media cannot supply.

Two examples:

  1. Blood agar — nutrient agar supplemented with whole sheep blood, supporting fastidious organisms such as Streptococcus and Pneumococcus, and showing haemolysis patterns useful for identification.
  2. Chocolate agar — heated, lysed blood agar, releasing NAD (factor V) and haemin (factor X), supporting fastidious organisms such as Haemophilus influenzae and Neisseria species.

(Other valid examples: Loeffler’s serum slope for Corynebacterium diphtheriae; Lowenstein-Jensen medium for Mycobacterium tuberculosis.)

  1. 1Bacterial Morphology and Physiology

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  26. 26Transport media2015 September (Supplementary) (2010 Scheme)Short Note · 2 marks
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  28. 28Transport media2021 February (Supplementary) (2010 Scheme)Short Note · 2 marks
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  30. 30Bacterial spore2025 August (Supplementary) (2010 Scheme)Short Note · 2 marks
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  32. 32Differences between exotoxin and endotoxin2014 September (Supplementary) (2010 Scheme)Short Note · 2 marks
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  38. 38Name two selective media2022 July (Supplementary) (2019 Scheme)1 mark
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