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Paper I — 2023 July (Supplementary) (2010 Scheme) — Q12

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Paper I
2023 July (Supplementary) (2010 Scheme) · 40 marks · 120 min

Question

Stages of bacterial growth curve.

Q122 marksShort Notes

Answer

The bacterial growth curve shows four phases when a bacterial culture is inoculated into fresh liquid medium:

  1. Lag phase — little/no increase in cell number, but cells are metabolically active, adapting to the new medium and synthesizing components needed for growth.
  2. Log (exponential) phase — cells divide at a constant, maximal rate, cell number increasing exponentially; the phase of choice for antibiotic susceptibility testing and most physiological studies, since cells are most uniform and metabolically active.
  3. Stationary phase — growth rate plateaus as new cell division equals cell death, due to nutrient depletion and waste accumulation; some organisms produce secondary metabolites and endospores during this phase.
  4. Decline (death) phase — viable cell count progressively falls as nutrient exhaustion and toxic waste accumulation cause cell death to exceed division.
  1. 1Bacterial Morphology and Physiology

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