Paper I
2022 July (Supplementary) (2019 Scheme) · 100 marks · 180 min

Question

Biological control for autoclave

Q171 marks

Answer

The biological control (indicator) for autoclave sterilization is a spore strip/vial containing spores of Geobacillus stearothermophilus, a highly heat-resistant organism, processed alongside the load; absence of growth on subsequent incubation confirms that true sterilizing conditions were achieved throughout the load — the most definitive control, since it directly demonstrates biological kill rather than merely confirming the machine reached the set physical parameters (as physical/chemical indicators do).

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