Paper II — 2023 January (Supplementary) (2019 Scheme) — Q14

Paper II

2023 January (Supplementary) (2019 Scheme) · 100 marks · 180 min

Question

Which organism is identified by Nagler’s reaction.

Q141 marks★✓

Answer

Nagler’s reaction identifies Clostridium perfringens — demonstrated on egg-yolk agar, where the organism’s alpha-toxin (lecithinase) produces an opalescent zone of precipitation around colonies (due to lecithin hydrolysis in the egg-yolk medium), which is specifically inhibited on the half of the plate pre-treated with C. perfringens type-specific antitoxin, confirming the identification.

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1. 1Culture Media and Methods

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