Paper I — 2022 October (Supplementary) (2010 Scheme) — Q4

Paper I

2022 October (Supplementary) (2010 Scheme) · 40 marks · 120 min

Question

Enriched media

Q42 marksShort Notes★✓

Answer

Basal (simple) media such as nutrient broth/agar support only non-fastidious organisms. Enriched media are basal media supplemented with extra growth factors — whole blood, lysed blood, serum, or egg — that fastidious organisms need but ordinary media cannot supply.

Examples: Blood agar (whole sheep blood — supports Streptococcus, Pneumococcus; also shows hemolysis pattern — beta/alpha/gamma), Chocolate agar (heated, lysed blood — releases NAD and hemin — supports Haemophilus influenzae, Neisseria), Loeffler’s serum slope (coagulated serum — Corynebacterium diphtheriae), Lowenstein-Jensen medium (egg-based — Mycobacterium tuberculosis).

Related Concepts

1. 1Culture Media and Methods

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10. 10Enrichment media.2013 April (2010 Scheme)Short Note · 2 marks
11. 11Enriched media2018 February (2010 Scheme)Short Note · 2 marks
12. 12Anaerobic culture methods.2017 February (2010 Scheme)Short Note · 2 marks
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